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KMID : 0352519940310010193
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Korea Univercity Medical Journal
1994 Volume.31 No. 1 p.193 ~ p.201
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A Study on the Ultrastructure and Distribution of vasoactive Intestinal Polypeptide
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Abstract
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Vasoactive intestinal polypeptide(VIP) is a 28-aminoacid peptide belonging to the
secretin family of peptides. It is distributed widely in the nervous system and in the
gastrointestinal tract. It has been detected immunocytochemically in certain amacrine cell of mammalian retinas examined so far. But little study has been done on the ultrastructures and distributions of the VIP immunoreactive neurons in the
retinas
of rodents.
This study was conducted to define ultrastructures of VIP immunoreactive neurons in 10 adult guinea pig retinas by immunocytochemical method using anti-VIP antiserum. Seven eyes were used for immunostaining of retinal wholemount preparations
and the
remaining three eyes for electronmicroscopical immunostaining.
VIP immunoreactive neurons were amacrine cells located in the inner nuclear layer(INL) adjacent to the inner plexiform layer(IPL). VIP immunoreactive amacrine cells gave rise to two to five primary processes, form which finer processes
emerged
and distributed forming dense plexuses in the IPL close to the INL. The spacing of the warp and weft in the plexuses of fibers was 8 to 10 um in diameter. The distribution of VIP immunoreactive neurons was throughout the retina. But the
cell
density was variable according to the regions of the retina ;
lowest values were found in the upper nasal quadrant (34.1±10.3/mm2), whereas a peak was observed in correspondence with the central portion (70.0±10.8mm2). VIP immunoreactive
neurons received synaptic inputs from unlabelled amacrine and flat cone bipolar cells, and
made output synapses onto the dendrites of ganglion cell in sublamina a of the IPL.
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KEYWORD
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